Friday, April 12, 2019 | 12.45 – 13.15
“Define the Tumor Microenvironment with CyTOF® technology and microfluidics solutions”Fludigm enables the comprehensive cellular and molecular profiling of the immune system and the tumor microenvironment with proven mass cytometry, Imaging Mass Cytometry™ (IMC™) and automated microfluidics solutions.Whether you seek to target new biomarkers and pathways or to optimize the effectiveness of checkpoint inhibitors, CAR T cells or cancer vaccines, Fluidigm can help you identify new insights to reach your next research breakthrough.
Roberto Spada, Business Development Specialist, Mass Cytometry, Fluidigm
“Discovery of candidate predictive biomarkers for therapeutic response to immune checkpoint inhibitors in melanoma.”
Significant progress has been made in the development of molecular diagnostics to predict therapeutic response to immune checkpoint inhibitors (ICI) in melanoma, however there remain many patients whose response is not accurately predicted. Stimulated by this challenge, we have collaborated with Dr. David Rimm at Yale University to evaluate the application of high plex spatially resolved protein analysis in FFPE specimens using GeoMx® Digital Spatial Profiling (DSP) to the discovery of candidate biomarkers for ICI response in melanoma. Using DSP, we have analyzed the spatial expression of 44 immune-related proteins across 60 ICI-treated patients. The expression patterns of a subset of the proteins were first validated through comparison to Quantitative Immunofluorescence. DSP analysis identified multiple markers that were predictive of prolonged progression free and/or overall survival. This study exemplifies the power of high-plex protein analysis in FFPE tumor specimens in the context of therapeutic response.
Douglas Hinerfeld, PhD. Principal Product Application Scientist. Nanostring Technologies
- AKOYA BIOSCIENCES
Björn Wendik will discuss Akoya’s portfolio of solutions for multiplexed immunofluorescence that span applications across biomarker discovery and clinical research. Attendees will get a quick overview of the latest enhancements to the Opal™ multiplex IHC kits, the Vectra® Polaris™ System and inForm™ software analysis package. Dr. Wendik will also give a quick overview of the CODEX® benchtop system which enables researches to image 40+ biomarkers in a tissue sample, using existing fluorescence microscopes.
Björn Wendik, PhD, Field Application Scientist, Akoya Biosciences, Inc.